Влияние сочетаний аминокислот и дипептидов на жизнеспособность культур тканей нервной и иммунной систем молодых и старых крыс
DOI:
https://doi.org/10.33910/2687-1270-2024-5-2-186-195Ключевые слова:
аминокислоты, дипептиды, жизнеспособность, органотипическая культура ткани, нервная система, иммунная системаАннотация
Одно из приоритетных направлений в современной биологии и медицине — изучение регуляторных механизмов повышения жизнеспособности многоклеточных систем. Многие из 20 кодируемых аминокислот стимулируют клеточную пролиферацию в органотипической культуре различных тканей экспериментальных животных. Также известно, что в основе физической жизнеспособности лежит скорость восстановления организма путем стимуляции клеток к пролиферации или апоптозу, осуществляемой под влиянием цитокинов и/или пептидов. В Санкт-Петербургском Институте биорегуляции и геронтологии была разработана технология выделения из различных органов и тканей телят полипептидных комплексов, влияющих на органотипическую культуру тканей экспериментальных животных. В состав полипептидных комплексов входят короткие пептиды — тетра-, три- и дипептиды, которые, являясь минорными компонентами исследованных полипептидных комплексов, обладают высокой биологической активностью. На основе этих данных может быть осуществлена разработка новых лекарственных препаратов, предназначенных для лечения и профилактики общих гериатрических синдромов (например, старческой астении), а также заболеваний различных органов с учетом принципа тканеспецифичности. Целью работы было исследование эффекта различных комбинаций L-аминокислот и дипептидов на жизнеспособность клеток в органотипической культуре ткани нервной и иммунной систем у крыс разного возраста.
Библиографические ссылки
ЛИТЕРАТУРА
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Fedoreyeva, L. I., Kireev, I. I., Khavinson, V. Kh., Vanyushin, B. F. (2011) Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Moscow), vol. 76, no. 11, pp. 1210–1219. https://doi.org/10.1134/S0006297911110022
Gao, C., Cao, N., Wang, Y. (2021) The coming of age for branched-chain amino acids. Journal of Cardiovascular Aging, vol. 1, no. 2. https://doi.org/10.20517/jca.2021.02
Gonçalves, E. M., Gomes-Marcondes, M. C. C. (2010) Leucine affects the fibroblastic Vero cells stimulating the cell proliferation and modulating the proteolysis process. Amino Acids, vol. 38, no. 1, pp. 145–153. https://doi.org/10.1007/s00726-008-0222-7
Ivanova, P. N., Surma, S. V., Shchegolev, B. F. et al. (2018) The effects of weak static magnetic field on the development of organotypic tissue culture in rats. Doklady Biological Sciences, vol. 481, no. 4, pp. 132–134. https://doi.org/10.1134/S0012496618040075
Kandil, H. M., Argenzio, R. A., Chen, W. et al. (1995) L-glutamine and L-asparagine stimulate ODC activity and proliferation in a porcine jejunal enterocyte line. American Journal of Physiology, vol. 269, no. 1, pp. G591–G599. https://doi.org/10.1152/ajpgi.1995.269.4.G591
Masuoka, N., Lei, C., Li, H., Hisatsune, T. (2021) Influence of imidazole-dipeptides on cognitive status and preservation in elders: A narrative review. Nutrients, vol. 13, no. 2, article 397. https://doi.org/10.3390/nu13020397
Nishikawa, G., Kawada, K., Hanada, K. et al. (2022) Targeting asparagine synthetase in tumorgenicity using patient-derived tumor-initiating cells. Cells, vol. 11, no. 20, article 3273. https://doi.org/10.3390/cells11203273
Ostfeld, I., Hoffman, J. R. (2023) The effect of β-alanine supplementation on performance, cognitive function and resiliency in soldiers. Nutrients, vol. 15, no. 4, article 1039. https://doi.org/10.3390/nu15041039
Rezaei, R., Wu, G. (2022) Branched-chain amino acids regulate intracellular protein turnover in porcine mammary epithelial cells. Amino Acids, vol. 54, no. 11, pp. 1491–1504. https://doi.org/10.1007/s00726-022-03203-y
Richardson, N. E., Konon, E. N., Schuster, H. S. et al. (2021) Lifelong restriction of dietary branched-chain amino acids has sex-specific benefits for frailty and life span in mice. Nature Aging, vol. 1, no. 1, pp. 73–86. https://doi.org/10.1038/s43587-020-00006-2
Vanyushin, B. F., Khavinson, V. Kh. (2016) Short biologically active peptides as epigenetic modulators of gene activity. In: W. Doerfler, P. Böhm (eds.). Epigenetics — a different way of looking at genetics. Cham: Springer Publ., pp. 69–90. http://dx.doi.org/10.1007/978-3-319-27186-6_5
Vasconcelos, P. R., Guimarães, A. B., Campelo, M W. et al. (2015) Preconditioning with L-alanyl-glutamine upon cerebral edema and hypocampus red neurons counting in rats subjected to brain ischemia/reperfusion injury. Acta Cirúrgica Brasileira, vol. 30, no. 3, pp. 199–203. https://doi.org/10.1590/S0102-865020150030000006
Yu, Q., Wang, X., Wang, L. et al. (2016) Knockdown of asparagine synthetase (ASNS) suppresses cell proliferation and inhibits tumor growth in gastric cancer cells. Scandinavian Journal of Gastroenterology, vol. 51, no. 10, pp. 1220–1226. https://doi.org/10.1080/00365521.2016.1190399
Zalomaeva, E. S., Ivanova, P. N., Chalisova, N. I. et al. (2020) Effects of weak static magnetic field and oligopeptides on cell proliferation and cognitive functions in different animal species. Technical Physics, vol. 65, no. 10, pp. 1585–1590. https://doi.org/10.1134/S1063784220100254
REFERENCES
Aftabuddin, M., Kundu, S. (2007) Hydrophobic, hydrophilic, and charged amino acid networks within protein. Biophysical Journal, vol. 93, no. 1, pp. 225–231. https://doi.org/10.1529/biophysj.106.098004 (In English)
Anisimov, V. N., Khavinson, V. Kh. (2010) Peptide bioregulation of aging: Results and prospects. Biogerontology, vol. 11, no. 2, рр. 139–149. https://doi.org/10.1007/s10522-009-9249-8 (In English)
Bender, D. A. (1983) Effects of a dietary excess of leucine on the metabolism of tryptophan in the rat: A mechanism for the pellagragenic action of leucine. British Journal of Nutrition, vol. 50, no. 1, pp. 25–32. https://doi.org/10.1079/bjn19830068 (In English)
Bilen, M., Ibrahim, P., Barmo, N. et al. (2020) Methionine mediates resilience to chronic social defeat stress by epigenetic regulation of NMDA receptor subunit expression. Psychopharmacology, vol. 237, no. 10, pp. 3007– 3020. https://doi.org/10.1007/s00213-020-05588-8 (In English)
Bisset, E. S., Howlett, S. E. (2022) The use of dietary supplements and amino acid restriction interventions to reduce frailty in pre-clinical models. Nutrients, vol. 14, no. 14, article 2806. https://doi.org/10.3390/nu14142806 (In English)
Blank, H. M., Reuse, C., Schmidt-Hohagen, K. et al. (2023) Branched-chain amino acid synthesis is coupled to TOR activation early in the cell cycle in yeast. EMBO Reports, vol. 24, no. 9, article e57372. https://doi.org/10.15252/embr.202357372 (In English)
Calabrese, V., Scuto, M., Salinaro, A. T. et al. (2020) Hydrogen sulfide and carnosine: Modulation of oxidative stress and inflammation in kidney and brain axis. Antioxidants, vol. 9, no. 12, article 1303. https://doi.org/10.3390/antiox9121303 (In English)
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Fedoreyeva, L. I., Kireev, I. I., Khavinson, V. Kh., Vanyushin, B. F. (2011) Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Moscow), vol. 76, no. 11, pp. 1210–1219. https://doi.org/10.1134/S0006297911110022 (In English)
Gao, C., Cao, N., Wang, Y. (2021) The coming of age for branched-chain amino acids. Journal of Cardiovascular Aging, vol. 1, no. 2. https://doi.org/10.20517/jca.2021.02 (In English)
Gonçalves, E. M., Gomes-Marcondes, M. C. C. (2010) Leucine affects the fibroblastic Vero cells stimulating the cell proliferation and modulating the proteolysis process. Amino Acids, vol. 38, no. 1, pp. 145–153. https://doi.org/10.1007/s00726-008-0222-7 (In English)
Ivanova, P. N., Surma, S. V., Shchegolev, B. F. et al. (2018) The effects of weak static magnetic field on the development of organotypic tissue culture in rats. Doklady Biological Sciences, vol. 481, no. 4, pp. 132–134. https://doi.org/10.1134/S0012496618040075 (In English)
Kandil, H. M., Argenzio, R. A., Chen, W. et al. (1995) L-glutamine and L-asparagine stimulate ODC activity and proliferation in a porcine jejunal enterocyte line. American Journal of Physiology, vol. 269, no. 1, pp. G591–G599. https://doi.org/10.1152/ajpgi.1995.269.4.G591 (In English)
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Nishikawa G., Kawada, K., Hanada, K. et al. (2022) Targeting asparagine synthetase in tumorgenicity using patient-derived tumor-initiating cells. Cells, vol. 11, no. 20, art. 3273. https://doi.org/10.3390/cells11203273 (In English)
Ostfeld, I., Hoffman, J. R. (2023) The effect of β-alanine supplementation on performance, cognitive function and resiliency in soldiers. Nutrients, vol. 15, no. 4, article 1039. https://doi.org/10.3390/nu15041039 (In English)
Rezaei, R., Wu, G. (2022) Branched-chain amino acids regulate intracellular protein turnover in porcine mammary epithelial cells. Amino Acids, vol. 54, no. 11, pp. 1491–1504. https://doi.org/10.1007/s00726-022-03203-y (In English)
Richardson, N. E., Konon, E. N., Schuster, H. S. et al. (2021) Lifelong restriction of dietary branched-chain amino acids has sex-specific benefits for frailty and lifespan in mice. Nature Aging, vol. 1, no. 1, pp. 73–86. https://doi. org/10.1038/s43587-020-00006-2 (In English)
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Zalomaeva, E. S., Ivanova, P. N., Chalisova, N. I. et al. (2020) Effects of weak static magnetic field and oligopeptides on cell proliferation and cognitive functions in different animal species. Technical Physics, vol. 65, no. 10, pp. 1585–1590. https://doi.org/10.1134/S1063784220100254 (In English)
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Copyright (c) 2025 Наталья Иосифовна Чалисова, Галина Анатольевна Рыжак, Екатерина Александровна Никитина, Артемий Владимирович Рубинский
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