The effect of amino acid and dipeptide combinations on the viability of nervous and immune tissue cultures in young and old rats
DOI:
https://doi.org/10.33910/2687-1270-2024-5-2-186-195Keywords:
amino acids, dipeptides, viability, organotypic culture, nervous system, immune systemAbstract
One of the priority areas of contemporary biology and medicine is the investigation of regulatory mechanisms that enhance the viability of multicellular systems. Many of the 20 coding amino acids are known to stimulate cell proliferation in organotypic cultures of various tissues in experimental animals. Additionally, the processes of recovery — whether through stimulation of cell proliferation or apoptosis, mediated by cytokines and/or peptides — are closely linked to cell viability. In this context, Saint Petersburg Institute of Bioregulation and Gerontology has developed a method to extract polypeptide complexes from various organs and tissues of young cattle. These complexes contain tetra-, tri-, and dipeptides, which, despite being minor components of the complexes, exhibit high biological activity. Based on these findings, novel therapeutic agents may be developed for the treatment and prevention of common geriatric syndromes, such as senile asthenia syndrome, as well as for addressing age-related pathologies with a focus on tissue specificity. The aim of this study was to evaluate the effects of different combinations of L-amino acids and dipeptides on cell viability in organotypic nervous and immune tissue cultures in rats of different ages.
References
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