The effect of systemic ozone administration on behavioral activity and blood prooxidant-antioxidant balance in rats
DOI:
https://doi.org/10.33910/2687-1270-2026-7-1-110-120Keywords:
ozone, blood, prooxidant-antioxidant balance, lipid peroxidation products, antioxidant defense system, behavioral activity in ratsAbstract
Ozone is a physiological factor whose biological activity depends on shifts in the prooxidant-antioxidant balance within the body. It initiates lipid peroxidation in biological membranes by acting as a source of reactive oxygen species via the ozone‑oxygen mixture. The aim of this study was to investigate the effects of systemic administration of ozone at different concentrations on blood prooxidant‑antioxidant balance and behavioral activity in rats. Male outbred white rats were divided into four groups: a control group that received daily intraperitoneal injections of 1 ml of 0.9% NaCl for 10 days, and three experimental groups that received NaCl with ozone at doses of 1, 10, and 100 μg/kg over the same period. Emotional and exploratory behaviors, levels of lipid peroxidation products, and markers of the antioxidant system were assessed. The intermediate ozone dose (10 μg/kg) acted as an optimal hormetic stimulus, inducing moderate activation of lipid peroxidation (as evidenced by increased levels of malondialdehyde and diene conjugates) accompanied by a compensatory increase in antioxidants, including glutathione, catalase, ceruloplasmin, and α‑tocopherol. Behaviourally, this dose significantly increased horizontal and vertical motor activity as well as exploratory behaviour. In contrast, the high dose (100 μg/kg) induced oxidative stress, suppressed antioxidant defences, and reduced behavioural activity, indicating toxicity and a shift toward passive behaviour. The low dose (1 μg/kg) produced no significant effects. Overall, ozone at a dose of 10 μg/kg effectively activates adaptive mechanisms, suppresses excessive free‑radical activity, and stimulates central nervous system functions.
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