Water-soluble C70 fullerene derivative as a regulator of the reactive oxygen species level in cultured human cells
DOI:
https://doi.org/10.33910/2687-1270-2021-2-4-463-470Keywords:
oxidative stress, fullerenes, reactive oxygen species, nanocomposites, DNAAbstract
Fullerene derivatives of C60 and C70 are being investigated as potential tools for drug delivery to the body. Additionally, these compounds have the ability to effectively bind reactive oxygen species (ROS) in solutions and can be used as radioprotectors and antioxidants. However, a study of water-soluble C60 fullerene previously showed that this compound is able not only to block ROS, but also to induce secondary oxidative stress associated with cell response to a significant decrease in ROS levels. In order to further analyse the response of human cells to the presence of fullerene in the medium, we investigated the effect of a water-soluble C70 fullerene derivative on cultured human skin fibroblasts. С70 fullerene in a non-toxic concentration causes transient oxidative stress, which manifested itself in a short-term increase in the cellular DNA oxidation level. Stress occurs 3 hours after fullerene-induced decrease in the antioxidant response gene NRF2 activity and an increase in the activity of the NOX4 gene, which encodes an enzyme catalysing hydrogen peroxide synthesis. 24 hours later, the level of DNA oxidation decreases to the control values. It is suggested that fullerenes can be used not only as antioxidants, but also as potential inducers of an adaptive response that increases cell survival under negative environmental influences.
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Copyright (c) 2022 Ekaterina A. Savinova, Larisa V. Kameneva, Elizaveta S. Ershova, Elena V. Proskurnina, Pavel E. Umriukhin, Ivan V. Rodionov, Olga А. Dolgikh, Olga A. Kraevaya, Pavel A. Troshin, Natalya N. Veiko, Svetlana V. Kostyuk
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