Structural and functional parameters of the intestine in rats with different nervous system excitability
DOI:
https://doi.org/10.33910/2687-1270-2022-3-1-110-117Keywords:
rat lines, nervous system excitability, small intestine structure, digestive enzymes, intestinal defense mechanismsAbstract
The paper reports the results of the study on structural parameters of the intestine and the activity of a number of intestinal membrane enzymes in rat lines with high and low thresholds of nervous system excitability (HP, LP, low excitable and highly excitable, respectively) under normal conditions. It was found that the number of enterocytes and intraepithelial lymphocytes on the villi of the ileum increased in HP rats compared to LP animals. These changes in HP rats were accompanied by an increase in the activity of maltase in the ileum mucosa. In addition, HP rats showed a reduction in the activity of alkaline phosphatase in the mucosa and chyme of the large intestine. Compared to the LP line, HP rats also showed an increase in the mass of chyme in this part of the intestine. The latter may indicate a higher sensitivity of the intestine to inflammation and a lower motor activity of the colon in HP rats. The study revealed patterns in the relationship between the genetically determined level of excitability of the nervous system and the structural and functional characteristics of the intestine in adult animals. It allows us to conclude that low excitability of the nervous system may be related to an increased risk of developing inflammatory bowel disease.
References
ЛИТЕРАТУРА
Вайдо, А. И., Ширяева, Н. В., Павлова, М. Б. и др. (2018) Селектированные линии крыс с высоким и низким порогом возбудимости: модель для изучения дезадаптивных состояний, зависимых от уровня возбудимости нервной системы. Лабораторные животные для научных исследований, № 3, с. 12–22. https://doi.org/10.29296/2618723X-2018-03-02
Груздков, А. А., Дмитриева, Ю. В., Алексеева, А. С. и др. (2018) Оценка уровня всасывания глюкозы в тонкой кишке крыс различных линий в естественных условиях. Журнал эволюционной биохимии и физиологии, т. 54, № 4, с. 271–277.
Дюжикова, Н. А., Даев, Е. В. (2018) Геном и стресс-реакция у животных и человека. Экологическая генетика, т. 16, № 1, с. 4–26. https://doi.org/10.17816/ecogen1614-26
Егорова, В. В., Никитина, А. А., Хюттер, Г. Ю. (1986) Сравнительная характеристика d- и p-форм некоторых собственно кишечных гидролаз у различных животных. В кн.: А. М. Уголев (ред.). Мембранный гидролиз и транспорт. Л.: Наука, с. 85–98.
Пирс, Э. (1962) Гистохимия. М.: Изд-во иностранной литературы, 964 с.
Тимофеева, Н. М., Груздков, А. А., Зильбер, Ю. Д. и др. (1986) Физиология и биохимия ферментных адаптаций. Тонкая кишка. В кн.: А. М. Уголев (ред.). Мембранный гидролиз и транспорт. Л.: Наука, с. 51–63.
Уголев, А. М. (1991) Теория адекватного питания и трофология. СПб.: Наука, 270 с.
Lallès, J.-P. (2014) Intestinal alkaline phosphatase: Novel functions and protective effects. Nutrition Reviews, vol. 72, no. 2, pp. 82–94. https://doi.org/10.1111/nure.12082
Lallès, J.-P. (2015) Intestinal alkaline phosphatase in stool: A novel biomarker for metabolic diseases. eBioMedicine, vol. 2, no. 12, p. 1866. https://doi.org/10.1016/j.ebiom.2015.12.001
Liu, W., Hu, D., Huo, H. et al. (2016) Intestinal alkaline phosphatase regulates tight junction protein levels. Journal of the American College of Surgeons, vol. 222, no. 6, pp. 1009–1017. https://doi.org/10.1016/j.jamcollsurg.2015.12.006
Malo, M. S. (2015) A high level of intestinal alkaline phosphatase is protective against type 2 diabetes mellitus irrespective of obesity. eBioMedicine, vol. 2, no. 12, pp. 2016–2023. https://doi.org/10.1016/j.ebiom.2015.11.027
REFERENCES
Dyuzhikova, N. A., Daev, E. V. (2018) Genom i stress-reaktsiya u zhivotnykh i cheloveka [Genome and stress-reaction in animals and humans]. Ekologicheskaya genetika — Ecological Genetics, vol. 16, no. 1, pp. 4–26. https://doi.org/10.17816/ecogen1614-26 (In Russian)
Egorova, V. V., Nikitina, A. A. Hütter, H. Yu. (1986) Sravnitel’naya kharacteristika d- i p-form nekotorykh sobstvenno kishechnykh gidrolaz u razlichnykh zhivotnykh [Comparative characteristics of d- and p-forms of some proper intestinal hydrolases in various animals] In: A. M. Ugolev (ed.). Membrannyj gidroliz i transport. Novye dannye i gipotezy [Membrane hydrolysis and transport. New data and hypotheses]. Leningrad: Nauka Publ., pp. 85–98. (In Russian)
Gruzdkov, A. A., Dmitrieva, Yu. V., Alekseeva, A. S. et al. (2018) Otsenka urovnya vsasyvaniya glyukozy v tonkoj kishke krys razlichnykh linij v estestvennykh usloviyakh [Evaluation of glucose absorption level in the small intestine of different rat strains under natural conditions]. Zhurnal evolyutsionnoj biokhimii i fiziologii — Journal of Evolutionary Biochemistry and Physiology, vol. 54, no. 4, pp. 271–277. (In Russian)
Lallès, J.-P. (2014) Intestinal alkaline phosphatase: Novel functions and protective effects. Nutrition Reviews, vol. 72, no. 2, pp. 82–94. https://doi.org/10.1111/nure.12082 (In English)
Lallès, J.-P. (2015) Intestinal alkaline phosphatase in stool: A novel biomarker for metabolic diseases. eBioMedicine, vol. 2, no. 12, p. 1866. https://doi.org/10.1016/j.ebiom.2015.12.001 (In English)
Liu, W., Hu, D., Huo, H. et al. (2016) Intestinal alkaline phosphatase regulates tight junction protein levels. Journal of the American College of Surgeons, vol. 222, no. 6, pp. 1009–1017. https://doi.org/10.1016/j.jamcollsurg.2015.12.006 (In English)
Malo, M. S. (2015) A high level of intestinal alkaline phosphatase is protective against type 2 diabetes mellitus irrespective of obesity. eBioMedicine, vol. 2, no. 12, pp. 2016–2023. https://doi.org/10.1016/j.ebiom.2015.11.027 (In English)
Pearse, E. (1962) Gistochimiya [Histochemistry]. Moscow: Foreign Literature Publ., 964 p. (In Russian)
Timofeeva, N. M., Gruzdkov, A. A., Zil’ber, Yu. D. et al. (1986) Fiziologiya i biokhimiya fermentnykh adaptatsyj. Tonkaya kishka [Physiology and biochemistry of enzymatic adaptations. Small intestine]. In: A. M. Ugolev (ed.). Membrannyj gidroliz i transport. Novye dannye i gipotezy [Membrane hydrolysis and transport. New data and hypotheses]. Leningrad: Nauka Publ., pp. 51–63. (In Russian)
Ugolev, А. М. (1991) Teoriya adekvatnogo pitaniya i trofologiya [Theory of adequate nutrition and Trophology]. Saint Petersburg: Nauka Publ., 270 p. (In Russian)
Vaido, А. I., Shiryaeva, N. V., Pavlova, M. B. et al. (2018) Selektirovannye linii krys s vysokim i nizkim porogom vozbudimosti: model’ dlya izucheniya dezadaptivnykh sostoyanij, zavisimykh ot urovnya vozbudimosti nervnoj sistemy [Selected rat strains HT, LT as a model for the study of dysadaptation states dependent on the level of excitability of the nervous system]. Laboratornye zhivotnye dlya nauchnykh issledovanij — Laboratory Animals for Science, no. 3, pp. 12–22. https://doi.org/10.29296/2618723X-2018-03-02 (In Russian)
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