Enhancement of stress-induced memory formation in rats: The role of collapsin-response mediator protein 2 (CRMP2)
DOI:
https://doi.org/10.33910/2687-1270-2024-5-4-388-395Keywords:
Wistar female rats, dominant model, stress, shuttle box, сollapsin-response mediator protein 2, indirect ELISA-testAbstract
The reported study investigates the impact of chronic stress on long-term memory formation and the expression of collapsin-response mediator protein 2 (CRMP2) within various brain structures in Wistar female rats. In the first experimental series, food-deprived rats were placed in pairs into a dominant model box for 5 minutes, over a period of five consecutive days. Dominant and submissive rats were categorized based on the amount of time spent at the feeder. In the second series, dominant and submissive rats were individually subjected to a 5-minute passive swimming test in a container of fresh water, with passive swimming duration recorded. Submissive rats exhibited a passive swimming time 2.5 times longer than that of dominant rats. In the third series, both groups were trained in a shuttle box for six consecutive days, with submissive rats achieving a greater number of correct trials compared to dominant rats. The levels of CRMP2 were measured in the amygdala, hippocampus, and left parietal cortex of both groups using a solid-phase indirect ELISA test. The results showed higher CRMP2 levels in the amygdala, hippocampus, and left parietal cortex of submissive rats compared to dominant rats. The upregulation of CRMP2 in the amygdala of submissive rats is hypothesized to be an adaptive response to chronic stress, while its increased expression in the hippocampus and parietal cortex is suggested to contribute to the stimulatory effect of stress on memory formation.
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