Possible physiological function of endogenous ouabain
DOI:
https://doi.org/10.33910/2687-1270-2021-2-1-96-101Keywords:
nociceptive neurons, NaV1.8 channels, patch-clamp method, organotypic nerve culture method, confocal laser scanning microscopy, endogenous ouabainAbstract
Our results suggest that endogenous ouabain triggers two different signaling processes. The first, fast process, modulates the activation gating device of the NaV1.8 channels, thereby reducing their functional activity. The second, slow process, decreases the density of NaV1.8 channels in the membrane of the primary sensory neuron. We assume that in this case, endogenous ouabain triggers a downstream cascade leading to a decrease in the expression of the SCN10A gene that produces NaV1.8 channels. It can be concluded that endogenous ouabain, when it interacts with the primary sensory neuron, performs important function of modulating functional activity of NaV1.8 channels. The practical result of the study was the assumption that the delivery of ouabain as a drug substance to the membrane of a nociceptive neuron in nanomolar concentration should lead to a safe and effective antinociceptive action of this agent at the organismal level.
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Copyright (c) 2021 Valentina A. Penniyaynen, Vera B. Plakhova, Svetlana A. Podzorova, Stanislav G. Terekhin, Boris V. Krylov
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