Expression of mtor and creb1 genes in the honeybee brain under the action of electromagnetic radiation of 2.4 GHz

Authors

  • Tatiana G. Zachepilo Pavlov Institute of Physiology, Russian Academy of Sciences https://orcid.org/0000-0001-6350-7050
  • Alisa K. Pribyshina Pavlov Institute of Physiology, Russian Academy of Sciences

DOI:

https://doi.org/10.33910/2687-1270-2024-5-3-325-330

Keywords:

honeybee, electromagnetic radiation, brain, CREB1, mTOR

Abstract

Electromagnetic radiation can potentially disrupt intracellular signaling pathways, thereby affecting cellular metabolism. Among the key regulators of gene activity in neurons are the transcription factor CREB1 and the mTOR protein involved in cellular metabolism. This study examines the effect of 2.4 GHz electromagnetic radiation, commonly emitted by Wi-Fi router on the expression of the mtor and creb genes in the brain of the honeybee (Apis mellifera L.). Honeybees, essential pollinators in ecosystems, are particularly vulnerable to electromagnetic radiation due to their interaction with environmental signals. In this experiment, honeybees in the experimental group were exposed to radiation for three hours, while the control group remained unexposed. After exposure, brain tissue was collected, and RNA was isolated for reverse transcription and PCR analysis. Results revealed altered expression of both mtor and creb1 genes in treated honeybees compared to controls, suggesting a potential disturbance in cellular metabolism within the nervous tissue. These findings highlight the need for further investigation into the mechanisms by which electromagnetic radiation affects honeybee brain function.

References

Balmori, A. (2021) Electromagnetic radiation as an emerging driver factor for the decline of insects. Science of The Total Environment, vol. 767, article 144913. https://doi.org/10.1016/j.scitotenv.2020.144913 (In English)

Boer, U., Noll, C., Cierny, I. et al. (2010) A common mechanism of action of the selective serotonin reuptake inhibitors citalopram and fluoxetine: Reversal of chronic psychosocial stress-induced increase in CRE/CREBdirected gene transcription in transgenic reporter gene mice. European Journal of Pharmacology, vol. 633, no. 1–3, pp. 33–38. https://doi.org/10.1016/j.ejphar.2010.01.016 (In English)

Dworkin, S., Mantamadiotis, T. (2010) Targeting CREB Signaling in Neurogenesis. Expert Opinion on Therapeutic Targets, vol. 14, no. 8, pp. 869–879. https://doi.org/10.1517/14728222.2010.501332 (In English)

Favre, D. (2011) Mobile phone-induced honeybee worker piping. Apidologie, vol. 42, no. 3, pp. 270–279. https://doi.org/10.1007/s13592-011-0016-x (In English)

Gehring, K. B., Heufelder, K., Feige, J. et al. (2016) Involvement of phosphorylated Apis mellifera CREB in gating a honeybee’s behavioral response to an external Stimulus. Learning & Memory, vol. 23, no. 5, pp. 195–207. https://doi.org/10.1101/lm.040964.115 (In English)

Ghiani, C. A., Beltran-Parrazal, L., Sforza, D. M. et al. (2007) Genetic program of neuronal differentiation and growth induced by specific activation of NMDA receptors. Neurochemical Research, vol. 32, no. 2, pp. 363–376. https://doi.org/10.1007/s11064-006-9213-9 (In English)

Halabi, N., Achkar, R., Haidar, G. A. (2013) The effect of cell phone radiations on the life cycle of honeybees. IEEE EUROCON 2013, pp. 529–536. https://doi.org/10.1109/EUROCON.2013.6625032 (In English)

Heberle, A. M., Prentzell, M. T., van Eunen, K. et al. (2014) Molecular mechanisms of mTOR regulation by stress. Molecular Cell Oncology, vol. 2, no. 2, article e970489. https://doi.org/10.4161/23723548.2014.970489 (In English)

Kazyken, D., Magnuson, B., Bodur, C. et al. (2019) AMPK directly activates mTORC2 to promote cell survival during acute energetic stress. Science Signaling, vol. 12, no. 585, article eaav3249. https://doi.org/10.1126/scisignal.aav3249 (In English)

Kida, S., Serita, T. (2014) Functional roles of CREB as a positive regulator in the formation and enhancement of memory. Brain Research Bulletin, vol. 105, pp. 17–24. https://doi.org/10.1016/j.brainresbull.2014.04.011 (In English)

Kumar, N. R., Sangwan, S., Badotra, P. (2011) Exposure to cell phone radiations produces biochemical changes in worker honey bees. Toxicology International, vol. 18, no. 1, pp. 70–72. https://doi.org/10.4103/0971-6580.75869 (In English)

Kuo, C. J., Hansen, M., Troemel, E. (2018) Autophagy and innate immunity: Insights from invertebrate model organisms. Autophagy, vol. 14, no. 2, pp. 233–242. https://doi.org/10.1080/15548627.2017.1389824 (In English)

Lopatina, N. G., Zachepilo, T. G., Kamyshev, N. G. et al. (2019) Vliyaniye neioniziruyushego elektromagnitnogo izlucheniya na povedeniye medonosnoj pchely Apis mellifera L. (Hymenoptera, Apidae) [Effect of non-ionizing electromagnetic radiation on behavior of the honeybee Apis mellifera L. (Hymenoptera, Apidae)]. Entomologicheskoye obozrenie — Entomological Rewiew, vol. 98, no. 1, pp. 35–43. https://doi.org/10.1134/S0367144519010039 (In Russian)

Migdal, P., Bieńkowski, P., Cebrat, M. et al. (2023) Exposure to a 900 MHz electromagnetic field induces a response of the honey bee organism on the level of enzyme activity and the expression of stress-related genes. PloS One vol. 18, no. 5, article e0285522. https://doi.org/10.1371/journal.pone.0285522 (In English)

Ortega-Martinez, S. (2015) A new perspective on the role of the CREB family of transcription factors in memory consolidation via adult hippocampal neurogenesis. Frontiers in Molecular Neuroscience, vol. 8, article 46. https://doi.org/10.3389/fnmol.2015.00046 (In English)

Ryskalin, L., Lazzeri, G., Flaibani, M. et al. (2017) mTOR-dependent cell proliferation in the brain. Biomedical Research International, vol. 2017, no. 1, article 7082696. https://doi.org/10.1155/2017/7082696 (In English)

Saliev, T., Begimbetova, D., Masoud, A.-R., Matkarimov, B. (2018) Biological effects of non-ionizing electromagnetic fields: Two sides of a coin. Progress in Biophysics and Molecular Biology, vol. 141, pp. 25–36. https://doi.org/10.1016/j.pbiomolbio.2018.07.009 (In English)

Tan, S., Wang, H., Xu, X. et al. (2021) Acute effects of 2.856 GHz and 1.5 GHz microwaves on spatial memory abilities and CREB-related pathways. Scientific Reports, vol. 11, no. 1, article 12348. https://doi.org/10.1038/s41598-021-91622-4 (In English)

Treder, M., Muller, M., Fellner, L. et al. (2023) Defined exposure of honey bee colonies to simulated radiofrequency electromagnetic fields (RF-EMF): Negative effects on the homing ability, but not on brood development or longevity. The Science of the total environment, vol. 896, article 165211. https://doi.org/10.1016/j.scitotenv.2023.165211 (In English)

Wang, X., Proud, C. G. (2006) The mTOR pathway in the control of protein synthesis. Physiology, vol. 21, pp. 362–369. https://doi.org/10.1152/physiol.00024.2006 (In English)

Wu, C. W., Storey, K. B. (2021) mTOR Signaling in metabolic stress adaptation. Biomolecules, vol. 11, no. 5, article 681. https://doi.org/10.3390/biom11050681 (In English)

Yamashima, T. (2012) “PUFA-GPR40-CREB Signaling” Hypothesis for the adult primate neurogenesis. Progress in Lipid Research, vol. 51, no. 3, pp. 221–231. https://doi.org/10.1016/j.plipres.2012.02.001 (In English)

Published

2024-11-29

Issue

Section

Experimental articles