Comparison of liquid and dry sucrose diets in the development of a model of metabolic syndrome in rats
DOI:
https://doi.org/10.33910/2687-1270-2023-4-3-373-378Keywords:
metabolic syndrome, sucrose diet, animal models, rat, insulin resistanceAbstract
The sucrose model, when sucrose is added to the animal’s diet as a part of drinking water, is widely used to model metabolic syndrome (MS) in rats. However, a sucrose solution is a favorable environment for the development of microorganisms and mold. The purpose of the reported study was to test the assumption that modeling MS in animals using a sucrose diet with the addition of lump sugar to the feed can serve as a simple and convenient alternative to dissolving sugar in drinking water. Male Wistar rats weighing 275–350 g were studied. During the experiment (14 weeks), the rats of the control group (C, n = 4) received standard dry food and drinking water. The rats of one experimental group (“liquid sugar”, LS, n = 4) had a free choice between drinking water or water sweetened with sugar (30% sugar;); the rats of the other experimental group (“solid sugar”, SS, n = 4) had a free choice between dry food and lump sugar (~30% of the total weight of food). It was revealed that the rats of the LS group consumed more sugar and calories than the rats of the SS group. Besides, the former developed glucose tolerance and insulin resistance faster. It is concluded that the sucrose diet using lump sugar, although more convenient to implement than the diet using sugar solution, is less effective in the development of MS in rats.
References
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Altman, D. G., Bland, J. M. (1999) How to randomise. British Medical Journal, vol. 319, no. 7211, pp. 703–704. https://doi.org/10.1136/bmj.319.7211.703 (In English)
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Cruz, E. M. S., de Morais, J. M. B., da Rosa, C. V. D. et al. (2020) Long-term sucrose solution consumption causes metabolic alterations and affects hepatic oxidative stress in Wistar rats. Biology Open, vol. 9, no. 3, article bio047282. https://doi.org/10.1242/bio.047282 (In English)
Derkach, K. V., Bondareva, V. M., Chistyakova, O. V. et al. (2015) The effect of long-term intranasal serotonin treatment on metabolic parameters and hormonal signaling in rats with high-fat diet/low-dose streptozotocin-induced type 2 diabetes. International Journal of Endocrinology, vol. 2015, no. 6, article 245459. https://doi.org/10.1155/2015/245459 (In English)
la Fleur, S. E., Luijendijk, M. C. M., van Rozen, A. J. et al. (2011) A free-choice high-fat high-sugar diet induces glucose intolerance and insulin unresponsiveness to a glucose load not explained by obesity. International Journal of Obesity, vol. 35, no. 4, pp. 595–604. https://doi.org/10.1038/ijo.2010.164 (In English)
Rodríguez-Correa, E., González-Pérez, I., Clavel-Pérez, P. I. et al. (2020) Biochemical and nutritional overview of diet-induced metabolic syndrome models in rats: What is the best choice? Nutrition & Diabetes, vol. 10, no. 1, article 24. https://doi.org/10.1038/s41387-020-0127-4 (In English)
Romanovsky, D., Walker, J. C., Dobretsov, M. (2008) Pressure pain precedes development of type 2 disease in Zucker rat model of diabetes. Neuroscience Letters, vol. 445, no. 3, pp. 220–223. https://doi.org/10.1016/j.neulet.2008.08.087 (In English)
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