Comparison of liquid and dry sucrose diets in the development of a model of metabolic syndrome in rats

Authors

DOI:

https://doi.org/10.33910/2687-1270-2023-4-3-373-378

Keywords:

metabolic syndrome, sucrose diet, animal models, rat, insulin resistance

Abstract

The sucrose model, when sucrose is added to the animal’s diet as a part of drinking water, is widely used to model metabolic syndrome (MS) in rats. However, a sucrose solution is a favorable environment for the development of microorganisms and mold. The purpose of the reported study was to test the assumption that modeling MS in animals using a sucrose diet with the addition of lump sugar to the feed can serve as a simple and convenient alternative to dissolving sugar in drinking water. Male Wistar rats weighing 275–350 g were studied. During the experiment (14 weeks), the rats of the control group (C, n = 4) received standard dry food and drinking water. The rats of one experimental group (“liquid sugar”, LS, n = 4) had a free choice between drinking water or water sweetened with sugar (30% sugar;); the rats of the other experimental group (“solid sugar”, SS, n = 4) had a free choice between dry food and lump sugar (~30% of the total weight of food). It was revealed that the rats of the LS group consumed more sugar and calories than the rats of the SS group. Besides, the former developed glucose tolerance and insulin resistance faster. It is concluded that the sucrose diet using lump sugar, although more convenient to implement than the diet using sugar solution, is less effective in the development of MS in rats.

References

ЛИТЕРАТУРА

Байрашева, В. К., Пчелин, И. Ю., Егорова, А. Э. и др. (2019) Экспериментальные модели алиментарного ожирения у крыс. Juvenis Scientia, № 9-10, с. 8–13. https://doi.org/10.32415/jscientia.2019.09-10.02

Altman, D. G., Bland, J. M. (1999) How to randomise. British Medical Journal, vol. 319, no. 7211, pp. 703–704. https://doi.org/10.1136/bmj.319.7211.703

Cruz, E. M. S., de Morais, J. M. B., da Rosa, C. V. D. et al. (2020) Long-term sucrose solution consumption causes metabolic alterations and affects hepatic oxidative stress in Wistar rats. Biology Open, vol. 9, no. 3, article bio047282. https://doi.org/10.1242/bio.047282

Derkach, K. V., Bondareva, V. M., Chistyakova, O. V. et al. (2015) The effect of long-term intranasal serotonin treatment on metabolic parameters and hormonal signaling in rats with high-fat diet/low-dose streptozotocin-induced type 2 diabetes. International Journal of Endocrinology, vol. 2015, no. 6, article 245459. https://doi.org/10.1155/2015/245459

la Fleur, S. E., Luijendijk, M. C. M., van Rozen, A. J. et al. (2011) A free-choice high-fat high-sugar diet induces glucose intolerance and insulin unresponsiveness to a glucose load not explained by obesity. International Journal of Obesity, vol. 35, no. 4, pp. 595–604. https://doi.org/10.1038/ijo.2010.164

Rodríguez-Correa, E., González-Pérez, I., Clavel-Pérez, P. I. et al. (2020) Biochemical and nutritional overview of diet-induced metabolic syndrome models in rats: What is the best choice? Nutrition & Diabetes, vol. 10, no. 1, article 24. https://doi.org/10.1038/s41387-020-0127-4

Romanovsky, D., Walker, J. C., Dobretsov, M. (2008) Pressure pain precedes development of type 2 disease in Zucker rat model of diabetes. Neuroscience Letters, vol. 445, no. 3, pp. 220–223. https://doi.org/10.1016/j.neulet.2008.08.087

REFERENCES

Altman, D. G., Bland, J. M. (1999) How to randomise. British Medical Journal, vol. 319, no. 7211, pp. 703–704. https://doi.org/10.1136/bmj.319.7211.703 (In English)

Bayrasheva, V. K., Pchelin, I. Yu., Egorova, A. E. et al. (2019) Eksperimental’nye modeli alimentarnogo ozhireniya u krys [Experimental models of alimentary obesity in rats]. Juvenis Scientia, no. 9-10, pp. 8–13. https://doi.org/10.32415/jscientia.2019.09-10.02 (In Russian)

Cruz, E. M. S., de Morais, J. M. B., da Rosa, C. V. D. et al. (2020) Long-term sucrose solution consumption causes metabolic alterations and affects hepatic oxidative stress in Wistar rats. Biology Open, vol. 9, no. 3, article bio047282. https://doi.org/10.1242/bio.047282 (In English)

Derkach, K. V., Bondareva, V. M., Chistyakova, O. V. et al. (2015) The effect of long-term intranasal serotonin treatment on metabolic parameters and hormonal signaling in rats with high-fat diet/low-dose streptozotocin-induced type 2 diabetes. International Journal of Endocrinology, vol. 2015, no. 6, article 245459. https://doi.org/10.1155/2015/245459 (In English)

la Fleur, S. E., Luijendijk, M. C. M., van Rozen, A. J. et al. (2011) A free-choice high-fat high-sugar diet induces glucose intolerance and insulin unresponsiveness to a glucose load not explained by obesity. International Journal of Obesity, vol. 35, no. 4, pp. 595–604. https://doi.org/10.1038/ijo.2010.164 (In English)

Rodríguez-Correa, E., González-Pérez, I., Clavel-Pérez, P. I. et al. (2020) Biochemical and nutritional overview of diet-induced metabolic syndrome models in rats: What is the best choice? Nutrition & Diabetes, vol. 10, no. 1, article 24. https://doi.org/10.1038/s41387-020-0127-4 (In English)

Romanovsky, D., Walker, J. C., Dobretsov, M. (2008) Pressure pain precedes development of type 2 disease in Zucker rat model of diabetes. Neuroscience Letters, vol. 445, no. 3, pp. 220–223. https://doi.org/10.1016/j.neulet.2008.08.087 (In English)

Downloads

Published

2023-10-31

Issue

Vol. 4 No. 3 (2023)

Section

Short Communications

License

Copyright (c) 2024 Yuri A. Filippov, Andrei V. Stepanov, Olga Yu. Karnishkina, Alexander A. Panov, Oksana V. Chistyakova, Maxim G. Dobretsov

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The work is provided under the terms of the Public Offer and of Creative Commons public license Creative Commons Attribution 4.0 International (CC BY 4.0).

This license permits an unlimited number of users to copy and redistribute the material in any medium or format, and to remix, transform, and build upon the material for any purpose, including commercial use.

This license retains copyright for the authors but allows others to freely distribute, use, and adapt the work, on the mandatory condition that appropriate credit is given. Users must provide a correct link to the original publication in our journal, cite the authors' names, and indicate if any changes were made.

Copyright remains with the authors. The CC BY 4.0 license does not transfer rights to third parties but rather grants users prior permission for use, provided the attribution condition is met. Any use of the work will be governed by the terms of this license.